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More Standard Microbiology Reagents
20x SSC
17.53% (w/v) sodium chloride and 8.86% (w/v) trisodium citrate were dissolved in
distilled water. The pH of the solution was adjusted to 7.0 with 5 M sodium hydroxide.
20x SSPE
17.53% (w/v) sodium chloride, 2.76% (w/v) sodium dihydrogen orthophosphate and 0.74%
(w/v) EDTA were dissolved in distilled water and adjusted to pH 7.4 with 1 M sodium
hydroxide.
10x TAE
For 1 l of 10x TAE, 48.4 g Tris, 11.42 ml glacial acetic acid and 20 ml of 0.5 M EDTA
pH 8.0 stock solution were made up to volume with distilled water.
10x TBE
For 1 l of 10x TBE, 108 g Tris, 55 g boric acid and 40 ml of 0.5 M EDTA pH 8.0 stock
were made up to volume with distilled water.
TE buffer
TE buffer (10 mM Tris, 1 mM EDTA) was made by mixing 200 µl of 1 M Tris·HCl pH 7.5
and 40 µl of 0.5 M EDTA pH 8.0, and making up to a volume of 20 ml with distilled water.
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