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Multiple Forms of Pectinesterase are Present in Tomato and Other Fruit

Strawberry

The dominant isoform (Graph 5, fractions 24-34) elutes from the column in a similar position to tomato PE2. Minor isoform(s) of PE in strawberry are just about visible in fractions 45-48, so these also elute from the column in much the same position as tomato PE1 and PE3. It could therefore be inferred that the PE isoforms of strawberry are similar to their tomato counterparts.

Levels of PE in strawberry increase during the green to early ripe stages of ripening. This, together with the apparent lack of other cell wall degrading enzymes such as polygalacturonase, could mean that PE has an important role in the ripening of strawberry.

Further Reading

• Title Page
• Introduction
• Assay development
• Total Pectinesterase activity in fruit
• Pectinesterase in Tomato
• Pectinesterase in Strawberry
• Pectinesterase in citrus fruits
• Pectinesterase in Grapes
• Summary & Future work
• References